FIG. 4.

Effect of MUC1 shRNA on integrin expression by fibroblasts. Neonatal fibroblasts were transfected with MUC1 shRNA (M) or scrambled shRNA (S) as described in Methods. (A) Transfected cells were lysed and analyzed by RT-PCR using primers for α₂-integrin (see Supplementary Table S1). (B) Transfected cells were lysed and analyzed by Western blotting (WB) using an antibody against α₂-integrin. GAPDH was used as a loading control. The bar chart shows densitiometric analysis of α₂-integrin protein bands from three separate experiments. Intensity values (relative to GAPDH) are given as means±SEM. The asterisk indicates that the value was significantly different (Student's t-test; p<0.05; n=3) from the scrambled control. (C) Transfected cells were analyzed by RT-PCR using a panel of primers against other integrins as indicated (see Supplementary Table S1).