Skip to main content
. Author manuscript; available in PMC: 2014 Apr 22.
Published in final edited form as: Curr Cell Biochem. 2011 Dec 28;1(1):20–32.

Figure 1.

Figure 1

Effect of insulin receptor kinase inhibitor on fusion between endosomes. (A) Effect of cytosol and energy on the endosome-endosome fusion. Five-min vesicles containing either Biotin-Insulin (B-Ins) or Avidin-β-Galactosidase (Av-GAL) were mixed in fusion buffer supplemented with different amount of cytosol containing ATP-regenerated (■) or ATP-depleted systems (●). Samples were then transferred to 37°C for the indicated times and processed as described in Material and Methods to determine the percentage forming the immune complex formation. The data are presented as means ± SD of four independent experiments. (B) Fusion assay was performed under standard conditions as described above either in the absence (□) or in the presence of different amounts of AG9 (○) and AG1024 (●) containing 1 mg/ml of cytosol. The data are presented as means ± SD of four independent experiments.