Figure 1.

Screen for Resistance to Differentiation Using Haploid Rex1GFPd2 Reporter ESCs

(A) Screening strategy used to identify regulators of the entry into differentiation.

(B) Proportion of reads in forward (FW) and reverse (REV) orientations within transcription units. Mean and standard deviation between all screens is shown.

(C) GO keyword analysis of all screen hits satisfying cutoff criteria.

(D) Gene list showing members of the Fgf/ERK pathway and the β-catenin/Tcf3 axis identified in at least one screen.

(E) List of 24 candidate genes that were subjected to siRNA validation. A plus sign denotes confirmation of a defect in the indicated assay.

(F) siRNA knockdown of a subset of candidate genes in diploid Rex1GFPd2 ESCs 24 hr after withdrawal of inhibitors.

See also Figure S1.