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The Sonic Hedgehog (Shh) signaling pathway components are detected in primary gastric cancer cells. Immunofluorescent staining was manipulated to investigate the expression of SHH, PTCH, SMO, GLI1, and GLI2 in human primary gastric cancer cells (red), and the nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 100 μm.
