Table 1.

Primers used in PCR for site directed mutagenesis of S. mutans NOX 2.

Mutated amino acidsa)	Forward primerb) (mismatched bases are underlined)
Asp192→Ala	5’-agaagttatcctgatcgccgttgttgacacctgcc-3’
Asp192→Arg	5’-aaagaagttatcctgatcaacgttgttgacacctgcc-3’
Val193→Arg	5’-gaagttatcctgatcgaccgtgttgacacctgcctggc-3’
Val194→His	5’-gttatcctgatcgacgttcatgacacctgcctggca-3’
Ala199→Arg	5’-gttgttgacacctgcctgcgtggttactacgaccaggac-3’
Gly200→Lys	5’-gttgacacctgcctggcaaaatactacgaccaggacctg-3’
Asp192→Ala/Val193→Arg	5’-taaagaagttatcctgatcgcccgtgttgacacctgcctggcag-3’
Val194→His/Ala199→Argc)	5’-gttcatgacacctgcctgcgtggttactacgaccaggac-3’
Val194→His/Gly200→Lysc)	5’-catgacacctgcctggcaaaatactacgaccaggacctg-3’

^a)Numbering refers to S. mutans NOX sequence beginning with 1 for the starting methionine

^b)reverse primers have reverse complementary sequence

^c)for combination of mutations the plasmid carrying the Val194→His mutation was used as template