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Apparent kinetic values and efficiencies for SmNOX wild type and variant 193R194H from purified protein.
| SmNOX variant | NADH | NADPH | ||||
|---|---|---|---|---|---|---|
|
| ||||||
| k cat (s-1) | K m (µM) | k cat/K m (s-1µM-1) | k cat (s-1) | K m (µM) | k cat/K m (s-1µM-1) | |
| Wild type | 36.8±0.3 | 7±2 | 5±2 | n.d. | n.d. | n.d. |
| 193R194H | 25.5±0.9 | 9±2 | 3±1 | 20±2 | 6±2 | 3±1 |
n.d. not detected
