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Influence of FAD and DTT on SmNOX, initial rates for NADH activity in 50 mM KPi set to 100%.
| SmNOX variant | Buffer only | +25µM FAD | +250µM FAD | +5mM DTT |
|---|---|---|---|---|
| Wild type | 100 | 108 | 112 | 104 |
| 193R194H | 100 | 89 | 87 | 110 |
SD from at least triplicate measurements was always <16%
