Skip to main content
. Author manuscript; available in PMC: 2014 Apr 22.
Published in final edited form as: Methods Mol Biol. 2013;1046:273–293. doi: 10.1007/978-1-62703-538-5_16

Fig. 1.

Fig. 1

Gel filtration chromatogram for pyrene labeled actin. An example of the UV absorbance detected chromatogram for pyrene labeled actin purification using a 320 mL Superdex 200 pg column. UV absorbance is shown with a thick black line. Fractions collected are shown with short vertical lines. Relevant fraction numbers are shown. The shape of the chromatographic peak is characteristic for actin purified using Superdex 200, and running the column in buffer G. For this purification, fractions 12, 13 and 14 were retained, and fractions 13 and 14 were used preferentially.