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. Author manuscript; available in PMC: 2014 Apr 22.
Published in final edited form as: Methods Mol Biol. 2013;1046:273–293. doi: 10.1007/978-1-62703-538-5_16

Fig. 2.

Fig. 2

Pyrene actin polymerization assay example. 4 μM actin, 5% pyrene labeled is brought from buffer G-Mg conditions to KMEI conditions to trigger polymerization of actin. Pyrene fluorescence intensity (Y-axis) as a function of time (X-axis) are shown for actin alone (thin line), actin in the presence of 10 nM bovine Arp2/3 complex (thick line) and actin in the presence of 10 nM bovine Arp2/3 complex and 250 nM N-WASP VCA (dashed line). These three curves are the controls for the basic behavior of actin and Arp2/3 complex.