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. Author manuscript; available in PMC: 2014 Apr 22.
Published in final edited form as: Oncogene. 2010 Oct 11;30(6):701–713. doi: 10.1038/onc.2010.441

Figure 7.

Figure 7

Apoptosis induced by CDDP/F-ara-A combination treatment is mediated by Noxa. (a) Protein expression levels of Noxa and actin in untransfected cells and in cells nucleofected with Noxa or control (scr) siRNA, untreated (left panels) or treated with 10 μm CDDP and 10 μm F-ara-A for 48 h (right panels), were analyzed by western blot for control of knockdown of Noxa. Apoptosis was assessed by MitoTracker staining (patient no. 1). (b) Protein expression levels in four patients (patient nos. 1–3 and 5), treated as described in (a) were quantified using Licor Odyssey software and the Noxa/Mcl-1 ratio was calculated. Noxa/Mcl-1 ratio of untransfected cells was set to 1. Bars represent mean + s.e.m. (*P<0.05, Mann–Whitney U-test). (c) Cells of p53-dysfunctional CLL patients were transfected with Noxa RNAi or control scrambled (Scr) RNAi and treated with 10 μm CDDP and 10 μm F-ara-A for 48 h, 100 μm CCCP for 24 h or left untreated. Apoptosis was assessed using annexin-V/PI staining (n = 4; patient nos. 1–3 and 5). Bars represent mean + s.e.m. (*P<0.05, Mann–Whitney U-test).