Fig. 4.
L-FABP deletion does not attenuate ATGL-mediated β-oxidation. Primary mouse hepatocytes were isolated from 8–10-week-old WT or L-FABP KO mice and transduced with Ad-GFP or Ad-ATGL for 24 h, at which time pulse (1.5 h) and chase (6 h) experiments were performed with 500 μM [1-14C]oleate. [1-14C]oleate incorporation into cell TG during the pulse period (A) and radiolabeled TG remaining after the chase period (C). ASMs from media were quantified at the end of pulse (B) and chase (D) periods. A pan-specific FABP inhibitor, HTS01037 (100 μM), was added during the chase period, and radiolabel incorporation into ASMs was quantified (E). ATGL-mediated fold induction was calculated as the Ad-ATGL values divided by the Ad-GFP values. Data are presented as mean ± SEM. *P < 0.05 versus GFP; #P < 0.05 versus WT mice; %P < 0.05 versus vehicle.