Fig. 1.

Gene expression of Angptl4. A: Mouse primary hepatocytes and H4IIE hepatoma cells were treated with control ethanol (EtOH), 0.5 µM Dex, 1 nM insulin, or Dex plus insulin, and in combination with one of the following inhibitors: 10 µM PI3K inhibitor GDC-0941, 5 µM Akt inhibitor API-2, 0.5 nM MK-8669 and 200 nM rapamycin, two TORC1 inhibitors, or 5 µM GSK inhibitor SB-216763. RNA isolation and RT-qPCR followed. Primers specific to Angptl4 and Rpl19 (internal control) gene were used in qPCR. Fold induction was calculated by normalizing to Rpl19 and taking the ratio of treatment over ethanol. Error bars indicate standard error of the mean. B: Schematic presentation of insulin signaling pathway in hepatocytes. Small-molecule inhibitors used to inhibit insulin signaling components are shown. C: Mouse primary adipocytes were treated with control ethanol (EtOH), 0.5 µM Dex, 1 nM insulin, or Dex plus insulin for 5 h. The expression of Angptl4 was assessed by RT-qPCR. Error bars indicate standard error of the mean. * P < 0.05 and n ≥ 3.