Fig. 5.

FoxO1 mediates insulin response on Dex-induced Angptl4 gene expression. H4IIE cells were infected with adenovirus carrying control LacZ (Ad-LacZ) or mutant FoxO1 lacking transactivation domain (Ad-FoxO1Δ256). A: Immunoblotting shows overexpression levels. B: After 48 h of infection, cells were treated with control ethanol, Dex, or a combination of Dex plus insulin for 6 h, followed by RT-qPCR for Angptl4 gene expression. Fold induction was calculated by normalizing to Rpl19 and taking the ratio of treatment over ethanol. Error bars indicate standard error of the mean. * P < 0.05; N.D. indicates no difference.