Comparison of HPLC chromatographic patterns of the bile acid 24-phenacyl esters. Typical HPLC chromatogram of the standard bile acid phenacyl esters (A), and the representative chromatographic patterns of the healthy control (B), early cirrhosis (C), and advanced cirrhosis (D). The analytical conditions are detailed in the Experimental Procedures. The number on each peak represents the compound number in the appendix: 1, CA; 2, CDCA; 3, DCA; 4, LCA; 5, UDCA; 8, isoDCA; 9, isoLCA; 11, HCA; 24, isoLCA-Δ5 ; 33, 7KetoDCA; 34, 7KetoLCA; 36, norDCA (IS); 35, LCA-3-one. *Unknown peaks but not bile acids confirmed by the GC-MS and LC-MS analysis.