TABLE 6.
Median concentrations (μmol/g) of all the tested BAs obtained by the LC-MS method
| Control (n = 10) (μmol/g) | Early (n = 17) (μmol/g) | Advanced (n = 23) (μmol/g) | ||
| Totala | 4.47 | 4.50 | 1.05 | |
| Subtotalb | 4.47 | 4.50 | 1.05 | |
| Unconjugated bile acids | ||||
| 1 | CA | 0.01 | 0.01 | 0.16 |
| 2 | CDCA | 0.00 | 0.00 | 0.10 |
| 3 | DCA | 1.87 | 1.05 | 0.01 |
| 4 | LCA | 1.48 | 0.91 | 0.00 |
| 5 | UDCA | 0.00 | 0.00 | 0.00 |
| 8 | isoDCA | 0.36 | 0.13 | 0.00 |
| 9 | isoLCA | 0.00 | 0.00 | 0.00 |
| 10 | allo-iso-LCA | 0.00 | 0.00 | 0.00 |
| 11 | HCA | 0.20 | 0.07 | 0.02 |
| Total unconjugated | 4.44 | 3.91 | 1.05 | |
| Percent total unconjugated (mean ± SD) | 97.4 ± 4.8 | 97.4 ± 4.3 | 81.2 ± 27.6 | |
| N-acylamidated (glycine and taurine) bile acids | ||||
| 39–48c | 0.00 | 0.00 | 0.00 | |
| Total amidated | 0.00 | 0.00 | 0.00 | |
| Percent total amidated (mean ± SD) | 0.1 ± 0.2 | 0.9 ± 2.4 | 0.3 ± 0.8 | |
| Sulfated bile acids | ||||
| 52 | DCA3S | 0.01 | 0.00 | 0.01 |
| 49–51,53c | 0.00 | 0.00 | 0.00 | |
| Total sulfated | 0.03 | 0.01 | 0.07 | |
| Percent total sulfated (mean ± SD) | 2.5 ± 4.7 | 1.7 ± 3.4 | 16.3 ± 25.6 | |
| Double conjugated bile acids | ||||
| 54–63c | 0.00 | 0.00 | 0.00 | |
| Total double conjugated | 0.00 | 0.00 | 0.00 | |
| Percent total double conjugated (mean ± SD) | 0.0 ± 0.0 | 0.0 ± 0.17 | 2.1 ± 6.4 | |
| Total conjugated | 0.03 | 0.01 | 0.08 | |
| Percent total conjugated (Mean ±SD) | 2.6 ± 4.8 | 2.7 ± 4.3 | 18.6 ± 27.3 |
a
Sum of all the bile acid species examined by this method.
b
Total of CA, CDCA, DCA, LCA, isoDCA, isoLCA, UDCA, HCA and their conjugated forms, which are common bile acid species examined by three different methods.
c
Bile acids denoted by the compound numbers are given in the Appendix.