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. Author manuscript; available in PMC: 2014 Apr 22.
Published in final edited form as: Reproduction. 2009 Aug 21;138(5):759–770. doi: 10.1530/REP-08-0535

Figure 1.

Figure 1

Effects of forskolin-pulsing on oocyte meiotic resumption. (A) Forskolin dose response. Denuded oocytes (DO), maintained in meiotic arrest with 1mM mbcAMP, were pulsed with increasing concentrations of forskolin for 3 h, washed free of forskolin, and cultures were continued in mbcAMP alone for 17-18 h before assessment of GVB. Other oocytes were continuously cultured with or without 100 μM forskolin in the meiotic-arresting medium for 20-21 h. Groups with no common letter are significantly different. (B) Maturation kinetics. Oocytes were treated as in (A) for 3 h; then, post-pulse cultures were carried out in medium drops under oil, with meiotic maturation assessed after the designated time periods.