Skip to main content
. Author manuscript; available in PMC: 2014 Apr 22.
Published in final edited form as: Reproduction. 2009 Aug 21;138(5):759–770. doi: 10.1530/REP-08-0535

Figure 2.

Figure 2

Effects of pulsing oocytes with forskolin and IBMX on oocyte maturation and PRKA activation. (A) DO were cultured for 21-22 h in 1 mM mbcAMP or mbcAMP plus forskolin (100 μM) and IBMX (100 μM); a third group was pulsed 3 h with forskolin plus IBMX, washed free of these agents and then cultured a further 17-18 h in mbcAMP alone before assessing meiotic status. Groups with no common letter are significantly different. (B) DO were exposed to the same treatments as in (A) except that GV-stage oocytes were collected after 5 h and extracts were processed for western analysis (300 oocytes per lane), using antibody to phospho-ACACA. The mean pACACA/ACACA ratio of four blots is shown, normalized to the mbcAMP alone group.