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. Author manuscript; available in PMC: 2014 Apr 22.
Published in final edited form as: Reproduction. 2009 Aug 21;138(5):759–770. doi: 10.1530/REP-08-0535

Figure 4.

Figure 4

Effects of 8-Br-cAMP pulsing on oocyte maturation. (A) Dose response effect of 8-Br-cAMP treatment. DO were pretreated for 3 h in 1 mM mbcAMP with increasing concentrations of 8-Br-cAMP before they were washed free of 8-Br-cAMP and cultured 17-18 h in mbcAMP alone. Separate sets of oocytes were cultured for 21-22 h in either mbcAMP alone or mbcAMP plus 4 or 6 mM 8-Br-cAMP. Groups with no common letter are significantly different. (B) Time-dependent effect of 8-Br-cAMP pretreatment. DO were pulsed with 6 mM 8-Br-cAMP for the indicated times before culture in 1 mM mbcAMP. The total culture time (including the pulsing time) was 20-21 h. Oocytes continuously cultured in mbcAMP or mbcAMP plus 6 mM 8-Br-cAMP for 20-21 h served as controls. Groups with no common letter are significantly different. (C) Effect of 8-Br-cAMP pulsing on HX-arrested oocytes. DO cultured in 4 mM HX were pulsed 3 h with 6 mM 8-Br-cAMP, washed free of the cyclic nucleotide and cultured 17-18 additional h in HX alone. Other oocytes were continuously cultured 21-22 h in HX alone or HX plus 8-Br-cAMP. Note that although the pulse group was not significantly different from the HX continuous group by ANOVA, it was by Student’s t-test, as indicated by the asterisk.