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. Author manuscript; available in PMC: 2014 Apr 22.
Published in final edited form as: Reproduction. 2009 Aug 21;138(5):759–770. doi: 10.1530/REP-08-0535

Figure 7.

Figure 7

Effects of PDE-resistant cAMP analog pulsing on oocyte maturation. mbcAMP-arrested DO were pretreated 3 h with increasing concentrations of Sp-cAMP-AM (A) or SP-5,6-DCI-cBIMPS (B) and then washed and cultured in mbcAMP alone for 17-18 h before assessment of GVB. Oocytes continuously cultured in mbcAMP or mbcAMP plus Sp-cAMP-AM (A) or SP-5,6-DCI-cBIMPS (B) for 20-21 h served as controls. Groups with no common letter are significantly different. (C) Western analysis of pPRKA and pACACA in SP-5,6-DCI-cBIMPS-pulsed GV-stage oocytes. After 3 h pretreatment in 1 mM mbcAMP plus 6 mM SP-5,6-DCI-cBIMPS, DO were washed and cultured 2 h in mbcAMP. DO cultured in mbcAMP or mbcAMP plus 200 μM SP-5,6-DCI-cBIMPS for 5 h served as controls. The mean pPRKA/ACACA and pACACA/ACACA ratios from 4 blots are shown, normalized to the mbcAMP alone group.