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. 2014 Mar 14;7:32. doi: 10.1186/1757-2215-7-32

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Copyright © 2014 Maurya et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Determination of expression and activity of Rac1 in DHEA induced PCOS ovary. Relative activity of Rac1 was analyzed in the DHEA (PCOS) and sham treated ovary (A). Expression level analysis of Rac1 was determined by immuno-blotting and densitometry in the ovary of PCOS and sham (B and C). The activity Rac1was assayed by analysis of its phosphorylated form (D and E).