Table 3.
qPCR reaction components
| Component | μl per reaction (1X) |
|---|---|
| TaqMan small RNA assay (20X) |
1 |
| Product from RT reaction/standard |
1.33 |
| TaqMan mastermix |
10 |
| Nuclease-free water |
7.67 |
| (Total) | (20) |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.