Skip to main content
. 2014 Apr 22;12(4):e1001844. doi: 10.1371/journal.pbio.1001844

Figure 4. TRiC knockdown reduces levels of activated Stat3 protein within cancer cells.

Figure 4

HS-578T cells (A) or HepG2 cells (E) stably transfected with lentivirus containing CCT2 shRNA (knockdown) or control shRNA (control) were immunoblotted with antibody to CCT2 or β-actin. Levels of activated Stat3 (Y705-phosphorylated Stat3, pStat3), total (t)Stat3, and GAPDH within cells lysates were measured using Luminex beads. Levels of pStat3 in HS-578T and HepG2 CCT2 or control knockdown cells are presented normalized to GAPDH (B and F) or tStat3 (D and H); levels of tStat3 in HS-578T and HepG2 CCT2- or control-knockdown cells are presented normalized to GAPDH (C and G). Results in CCT2 knockdown cells indicated with an asterisk (*) differ from control knockdown cells (p<0.02). The results shown are representative of two experiments.