Table 1.
Description of nucleotide start/stop locations and amino acid length of ORFs in SSME, along with predicted functions
| |
Start/stop (#aa) |
|
|---|---|---|
| ORF | SSME | Predicted function/conserved domains |
| 1R |
272-1042 |
Replication factor &/or DNA packing protein |
| 255aa | ||
| 2L |
1649-2629 |
Myristylated membrane protein, DUF230 poxvirus protein, TM* |
| 325aa | ||
| 3R |
3436-4752 |
IIV6 ORF 229L, SP |
| 437aa | ||
| 4R |
4793-4975 |
TM |
| 59aa | ||
| 5R |
5408-6022 |
FPV ORF 250, US22, herpes virus early nuclear protein |
| 203aa | ||
| 6R |
6025-6252 |
SP* |
| 74aa | ||
| 7R |
7043-7429 |
SP |
| 127aa | ||
| 8R |
7521-11402 |
DNA-dependent RNA polymerase largest subunit |
| 1292aa | ||
| 9L |
11771-14617 |
NTPase, SNF2 family, N-terminal, helicases C-terminal, DEAD/H helicases |
| 947aa | ||
| 10R |
14633-15046 |
TM |
| 136aa | ||
| 11R |
15396-15608 |
TM |
| 69aa | ||
| 12L |
15674-16567 |
Unknown protein, SP |
| 296aa | ||
| 13R |
17108-17314 |
SP |
| 67aa | ||
| 14R |
17329-17688 |
Unknown protein, SP |
| 118aa | ||
| 15R |
17784-18752 |
AAA-ATPase, poxvirus A32 |
| 321aa | ||
| 16R |
19032-19859 |
Integrase homologue |
| 274aa | ||
| 17L |
20100-21608 |
SP |
| 501aa | ||
| 18 L |
21645-21881 |
TM |
| 77aa | ||
| 19R |
21933-24515 |
Similar to LCDV1 or f10L conserved uncharacterized protein, SP |
| 859aa | ||
| 20R |
24562-25008 |
Unknown protein, TM |
| 147aa | ||
| 21L |
25231-25890 |
ISKNV ORF 56L-like protein |
| 218aa | ||
| 22R |
26020-28941 |
D5 family NTPase, ATPase |
| 972aa | ||
| 23R |
29319-30467 |
SP |
| 381aa | ||
| 24Rº |
30877-31947 |
SP |
| 355aa | ||
| 25R |
32141-32929 |
P31K protein |
| 261aa | ||
| 26R |
32996-33226 |
Truncated elf-2α homologue |
| 75aa | ||
| 27R |
33757-36669 |
Tyrosine kinase, CAP 10, putative lypopolysaccharide modifying enzyme |
| 969aa | ||
| 28R |
36718-37206 |
Unknown protein, SP |
| 161aa | ||
| 29L |
37385-37681 |
SP |
| 97aa | ||
| 30R |
37883-38035 |
TM |
| 49aa | ||
| 31R |
38097-38516 |
SP |
| 138aa | ||
| 32R |
38566-40458 |
Neurofilament triplet H1 protein |
| 629aa | ||
| 33R |
40541-40732 |
Unknown protein, TM |
| 62aa | ||
| 34R |
40875-41195 |
Human parainfluenza virus 1L like protein, TM |
| 105aa | ||
| 35L |
41287-41748 |
SP |
| 152aa | ||
| 36L |
41761-42384 |
SP |
| 206aa | ||
| 37R |
42778-43413 |
NIF/NLI interacting factor |
| 210aa | ||
| 38R |
43554-45251 |
Ribonucleoside diphosphate reductase alpha subunit barrel domain |
| 564aa | ||
| 39R |
45357-45707 |
Hydrolase of the metallo-beta-lactamase superfamily |
| 115aa | ||
| 40R |
45795-46343 |
TM |
| 181aa | ||
| 41R |
46725-50222 |
RRV ORF-2-like protein, SP |
| 1164aa | ||
| 42L |
50718-50975 |
SP |
| 84aa | ||
| 43R |
50974-51491 |
TM |
| 171aa | ||
| 44R |
51512-51697 |
SP |
| 60aa | ||
| 45L |
51973-52383 |
LCDV1 ORF-88-like protein, SP |
| 135aa | ||
| 46L§ |
52437-53000 |
RGI 47L-like protein, SSTIV 049L-like protein, Neurofilament triplet H1-like protein, microneme/rhoptry antigen |
| 186aa | ||
| 47L |
53125-53541 |
SP |
| 137aa | ||
| 48L |
53544-53795 |
SP |
| 82aa | ||
| 49/50L¶ |
53904-55451 |
LCDV1 ORF 58-like protein, RGI ORF 50L-like protein, SSTIV ORF 052-like protein, SAP DNA binding domain |
| 514aa | ||
| 51R |
55531-57216 |
Unknown, SP |
| 560aa | ||
| 52L |
57473-58540 |
3-beta-hydroxy-delta 5-C27 steroid oxidoreductase-like protein, TM |
| 354aa | ||
| 53R |
58878-60446 |
LCDV1 ORF-20-like protein, SP |
| 521aa | ||
| 54L |
60677-60907 |
Nuclear calmodulin-binding protein |
| 75aa | ||
| 55L |
60945-62240 |
Helicase-like protein |
| 430aa | ||
| 55R |
61089-62228 |
FV3 40-kDa protein, SP |
| 378aa | ||
| 56R |
62328-62765 |
SP |
| 144aa | ||
| 57R |
62879-64375 |
Phosphotransferase, S-TKc, Serine/Threonine protein kinase |
| 497aa | ||
| 58R |
64700-65413 |
SP |
| 236aa | ||
| 59L |
65964-67022 |
RGV 9807 unknown protein, SP |
| 351aa | ||
| 60R |
67184-70225 |
DNA polymerase-like protein, DNA polymerase family B exonuclease |
| 1012aa | ||
| 61L |
70234-70416 |
SP |
| 59aa | ||
| 62L |
70859-74524 |
DNA-directed RNA polymerase II second largest subunit RNA polymerase domain 6, 7, 3, 2 beta subunit |
| 1220aa | ||
| 63R |
74903-75397 |
dUTPase-like protein |
| 163aa | ||
| 64R |
75522-75809 |
Interleukin-1 beta convertase precursor, Caspase-recruitment domain/DEATH |
| 94aa | ||
| 65L# |
N/A |
SP |
| 66L# |
76106-76157 |
SP |
| 16aa | ||
| 67L |
76212-77375 |
Ribonucleoside-reductase diphosphate beta subunit-like protein |
| 386aa | ||
| 68R |
77657-77944 |
SP |
| 94aa | ||
| 69R |
78080-78346 |
Unknown protein, TM |
| 87aa | ||
| 70R |
78364-78738 |
SP |
| 123aa | ||
| 71R |
78778-79011 |
SP |
| 76aa | ||
| 72L |
79068-79784 |
SP |
| 238aa | ||
| 73L |
80232-81206 |
NTPase/helicase-like protein |
| 323aa | ||
| 74L |
81381-82493 |
SP |
| 369aa | ||
| 75L |
82525-82779 |
LITAF/PIG7 possible membrane associated motif in LPS-induced tumor necrosis factor alpha factor, TM |
| 83aa | ||
| 76R |
82842-83063 |
SP |
| 72aa | ||
| 77L |
83060-83407 |
LCDV ORF 2-like protein, SP |
| 114aa | ||
| 78L |
84007-84645 |
SP |
| 211aa | ||
| 79R |
84781-86499 |
ATPase-dependent protease |
| 571aa | ||
| 80L |
87122-88237 |
Ribonuclease III-like protein |
| 370aa | ||
| 81R |
88293-88571 |
Transcription elongation Factor SII, C2C2 zinc finger |
| 91aa | ||
| 82R |
88700-89173 |
Immediate-early protein ICP-18 |
| 156aa | ||
| 83R |
89623-90267 |
Cytosine DNA methyl-transferase |
| 213aa | ||
| 84R |
90652-91389 |
LCDV 1-like proliferating cell nuclear antigen, SP |
| 244aa | ||
| 85R |
91464-92051 |
Deoxynucleoside kinase |
| 194aa | ||
| 86L |
92441-92626 |
SP |
| 60aa | ||
| 87L** |
92979-94778 |
Unknown protein, SP |
| 598aa | ||
| 88R |
94811-95263 |
Evrl-air-augmenter of liver regeneration |
| 149aa | ||
| 89R |
95331-96497 |
SP |
| 388aa | ||
| 90R |
96590-97981 |
Major capsid protein |
| 462aa | ||
| 91R |
98105-99292 |
Immediate-early protein ICP-46 |
| 394aa | ||
| 92R |
99627-99872 |
SP |
| 80aa | ||
| 93L |
100054-100221 |
SP |
| 54aa | ||
| 94L |
100331-100798 |
Regina ranavirus P8.141 C-like protein, TM |
| 154aa | ||
| 95R |
100891-101982 |
DNA repair protein RAD2, Xeroderma pigmentosum G N-region, Helix-hairpin-helix, Class 2 (Pol I) family |
| 362aa | ||
| 96R |
102784-103455 |
Unknown protein, SP |
| 222aa | ||
| 97R |
103538-103951 |
Myeloid cell leukemia protein, MCL-1, TM |
| 136aa | ||
| 98R | 104649-104849 |
SP |
| 65aa |
*Note: SP-surface protein, TM-transmembrane domain.
ºLoss of original, predicted start codon in SSME; new start codon 27 bp downstream.
§Loss of original, predicted stop codon in SSME; new stop codon 319 bp upstream.
¶39 bp deletion in original 50L ORF of SSME. Loss of ORF 50L’s original, predicted stop codon in SSME; ORFs 49L and 50L are now combined as 49/50L.
#Deletion of 65L ORF and majority of 66L ORFs in SSME.
**18 bp deletion in 87L ORF of SSME.