Table 2.

Genetic variation present within the SSME genome as compared to the wt-FV3 reference genome

Region	Change	Affect
21,933-24,515 bp	24 bp substitutions	24aa substitutions & 9aa insertion in 19R
	27 bp insertion
30,851 bp	1 bp substitution	Loss of a start codon in 24R; new start codon is 27 bp downstream, thus shortening ORF by 9aa
51,102 bp	1 bp deletion	Frameshift mutation in 43R
52,769 bp	1 bp deletion	Frameshift mutation and loss of original stop codon in 46L; new stop codon is 319 bp upstream, thus lengthening 46L
54,805 bp	1 bp deletion	Loss of original stop codon in 50L, leading to the combination of ORFs 49L and 50L
54,938 bp	39 bp deletion	13aa deletion in 49/50L
76,105 bp	757 bp deletion	Deletion of all of 65L and the majority of 66L
94,712 bp	18 bp deletion	6aa deletion in 87L
10,4050 bp	67 bp deletion	67 bp deletion in non-coding region between 97R and 98R

Genome region involved, nucleotide change, and end affect of the variation is presented.