Table 1. Bacterial strains and plasmids used in this study.
| Strain or plasmid | Characteristic(s) | Reference |
| Strains | ||
| E. coli | ||
| GeneHogs | Invitrogen | |
| EPI300 | Epicentre | |
| E. faecalis | ||
| V583 | [58] | |
| OG1RF | [59] | |
| TX5179 | OG1RF ΔepaB | [41] |
| TX5180 | OG1RF ΔepaE | [41] |
| OU510 | Contains an amber mutation in fsrD, resulting in a lack of GBAP biosynthesis | [20] |
| LMGT3690 | V583ΔgelE | Diep, Hernandez and Nes, unpublished |
| MS232 | V583 pAT28p11fsrD | This study |
| MS234 | V583ΔgelE pAT28p11fsrD | This study |
| MS253 | OU510 empty pAT28 | This study |
| MS269 | OU510 pREG696-PfsrB-luxABCDE | This study |
| MS272 | OU510 pREG696-PgelE-luxABCDE | This study |
| MS377 | TX5179 pAT28epaBCD with native promoter | This study |
| MS381 | TX5179 empty pAT28 | This study |
| MS383 | OG1RF empty pAT28 | This study |
| Plasmids | ||
| pCC1 | Single-copy cloning vector, camR | Epicentre |
| pAT28 | Shuttle vector, specR | [13] |
| pAT28pCC1 efaBCD | efaBCD and their native promoter inserted in pAT28 | This study |
| pAT28 P11 fsrD | fsrD and the P11 promoter inserted in pAT28 | This study |
| pLei1 | Leanti La Rosa, unpublished | |
| pREG696 | Low-copy-number vector, stable due to axe-txe toxin-antitoxin locus, specR | [18] |
| pREG696-PfsrB-luxABCDE | This study | |
| pREG696-PgelE-luxABCDE | This study | |
| pSL101P16S | pPL2lux derivative containing a synthetic P16S promoter and the axe-txecassette, specR | [19] |
Amp = ampicillin, cam = cloramphenicol, ery = erythromycin, r = resistance and spec = spectinomycin.