Table 5.
Specifications of oligonucleotides used for RT-qPCR 1-3
| Gene symbol | Full gene name | Acc. Number 1 | Primer pairs (5′-3′) | Amplicon length |
|---|---|---|---|---|
| CPT1A |
carnitine palmitoyltransferase 1A |
XM_002699420.2 |
F: TTCTTCTGGGGTCTACGATTCC |
119 |
| |
|
|
R: GATGTGCTTGCTGTCCCTCAG |
|
| DGAT1 |
diacylglycerol O-acyltransferase 1 |
NM_174693.2 |
F: TTGGCAGGTAAGGCGGC |
99 |
| |
|
|
R: GGGGGCGAAGAGGAAGTAGT |
|
| ELOVL2 |
fatty acid elongase 2 |
NM_001083517.1 |
F: GTCTTCTTACATGATGACGCTGGT |
72 |
| |
|
|
R: ATTGGCTTTTTCCGGTATGTCTGA |
|
| ELOVL5 |
fatty acid elongase 5 |
NM_001046597.1 |
F: CCCTCTCGGTTGGTTGTATTTC |
127 |
| |
|
|
R: GTGGTCCTTTTGGTGCTCTCTC |
|
| FADS1 |
fatty acid desaturase 1 |
XM_002699285.2 |
F: GTGGGTGGACTTGGCCTG |
103 |
| |
|
|
R: TGGGGCTTGTCTTCATGGTC |
|
| FADS2 |
fatty acid desaturase 2 |
NM_001083444.1 |
F: CGGCAAGAAGAAGCTGAAATACCTG |
92 |
| |
|
|
R: CTGCTCATCCCTTTGTATTTCCA |
|
| FASN |
fatty acid synthase |
NM_001012669.1 |
F: ATGGCGTTCCACTCCTACTTCA |
137 |
| |
|
|
R: CTCTCCTGCCACTGGGTCTC |
|
| INSR |
insulin receptor |
XM_002688832.2 |
F:ACGCTGGTGGTGATGGAGTT |
133 |
| |
|
|
R: TCTCTGCCGCCATCTGAATC |
|
| PPARA |
peroxisome proliferator-activated receptor alpha |
NM_001034036.1 |
F: CCAACAACAACCCGCCTTT |
125 |
| |
|
|
R: CGTCTTCTCGGCCATACACA |
|
| SCD |
stearoyl-CoA desaturase 9 |
NM_173959.4 |
F: CCATCAACCCCCGAGAGAAT |
76 |
| |
|
|
R: AAGGTGTGGTGGTAGTTGTGGAA |
|
| SREBF1c |
sterol regulatory element binding transcription factor 1 |
NM_001113302.1 |
F: ATCTCTTGGAGCGAGCACTGA |
115 |
| |
|
|
R: AGGTACCCCAGGGCATCTG |
|
| RPS93 |
ribosomal protein S9 |
NM_001101152.1 |
F: GAAGGTAATGCCCTGTTGCG |
141 |
| |
|
|
R: CAGGCCCAGCTTGAAGACC |
|
| SDHA3 |
succinate dehydrogenase complex subunit A |
NM_174178.2 |
F: TGCAGGAAGGCTGTGAGAAGAT |
100 |
| R: GTCTCCACCAGGTCAGTGTTCC |
1Entrez Gene, National Center for Biotechnology Information (NCBI).
2F: forward primer and R: reverse primer.
3Housekeeping gene.