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. 2014 Jan 30;7:e27683. doi: 10.4161/cib.27683

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Figure 1. Isolation of bacterial strain and in vitro characterization for their PGPR traits. Clear halo zone on Pikovskaya’s agar and Skimmed milk agar plate showing substrate solubilization (A) and Caseins hydrolyzation (B). Phosphate solublizing data using the calibration curve with KH2PO4 at 600nm. The pH variation in the Pikovskaya’s medium during growth of isolates at different time points (C). Productions of indole-3-acetic supplemented with tryptophan 250 µg/mLand 500 µg/mL compared with absence of tryptophan (D). Thin layer chromatography (TLC) of indole-3-acetic acid detected by Salkowiski reagent (E).