Skip to main content
. Author manuscript; available in PMC: 2014 Apr 22.
Published in final edited form as: Circ Res. 2013 Mar 21;112(12):1602–1612. doi: 10.1161/CIRCRESAHA.112.300648

Figure 4. Overexpression of miR-144 in vivo Reduces both Hepatic ABCA1 Protein and Plasma HDL Cholesterol Levels.

Figure 4

(A–D) Wild-type C57BL/6 mice (8–10/group) were infected with Ad-control or Ad-pre-miR-144. After 5 days, (A) Hepatic mature miR-144 was determined by Taqman-based RT-qPCR. In addition, (B) Abca1 mRNA and protein were determined SYBR green RT-qPCR and Western blotting respectively. Insert shows representative Western blot and protein changes were quantified by densitometry after normalization to β-actin (n=5/group). (C) Plasma total and HDL cholesterol, (D) plasma lipoprotein profiles determined by FPLC were determined as described in the Methods section. Data are shown as mean + SEM. Significance was determined using student t-test. *p<0.05, **p<0.01.