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. Author manuscript; available in PMC: 2015 May 16.
Published in final edited form as: Neuroscience. 2014 Mar 5;267:102–113. doi: 10.1016/j.neuroscience.2014.02.033

Figure 6.

Figure 6

Effect of TD on UCP1 expression in the brown adipose tissue. Female C57BL6 mice were fed with either control or TD diet. From the 26th day on, mice in TD+T group were changed to a normal liquid diet supplemented with additional benfotiamine. The mice were sacrificed and perfused on the 26th day in the TD group and the 33rd day in the TD+T and control groups, respectively. A. The expression of mitochondrial UCP1 (arrows) in the brown adipose tissue of mice in control, TD and TD+T group was demonstrated by IHC. The cells were counterstained with hematoxylin (arrowheads). Scale bar = 200 μm. B. The expression of UCP1 in the brown adipose tissue of control, TD, and TD+T mice was examined by immunoblotting. The change in the expression was quantified by densitometric analysis. *p<0.05 vs. control, # p<0.05 vs. TD, n = 6 per group.