Table 1. Target genes, primers and PCR reaction conditions used for strain characterization.

Target	Primer	Sequence (5′-3′)	Annealing temperature (°C)	Product size (bp)	Reference
Detection primers
qnrA	QnrAm-F	AGAGGATTTCTCACGCCAGG	54	516	[39]
	qnrA_R	GCCATACCTACGGCGATACC			[40]
qnrB	qnrB_F	GATCGTGAAAGCCAGAAAGG	54	476	[41]
	qnrB_R	ATGAGCAACGATGCCTGGTA			[41]
qnrC	qnrC-F	GGGTTGTACATTTATTGAATC	47	447	[6]
	qnrC-R	TCCACTTTACGAGGTTCT			[6]
qnrD	qnrD fw	CGAGATCAATTTACGGGGAATA	54	582	[7]
	qnrD rev	AACAAGCTGAAGCGCCTG			[7]
qnrS	QnrSm-F	GCAAGTTCATTGAACAGGGT	54	428	[39]
	QnrSm-R	TCTAAACCGTCGAGTTCGGCG			[39]
aac(6′)-Ib-cr	aac(6′)-Ib_For	TTGCGATGCTCTATGAGTGGCTA	55	482	[42]
	aac(6′)-Ib_Rev	CTCGAATGCCTGGCGTGTTT			[42]
qepA	QEPfor	TGGTCTACGCCATGGACCTCA	56	1137	[43]
	QEPrev	TGAATTCGGACACCGTCTCCG			[43]
Sequencing primers
gyrA	gyrA WF	AAATCTGCCCGTGTCGTTGGT	55	344	[41]
	gyrA WR	GCCATACCTACGGCGATACC			[41]
gyrB	gyrB-F	GAAATGACC CGCCGTAAA	55	272	[44]
	gyrB-R	ACGACCGATACCACAGCC			[44]
parC	parC WF	CTGAATGCCAGCGCCAAATT	55	168	[41]
	parC WR	GCGAACGATTTCGGATCGTC			[41]
parE	parE-F	CTGAACTGCTGGCGGAGATG	60	483	[44]
	parE-R	GCGGTGGCAGTGCGACGTAA			[44]
qnrS	qnrS1_orf_f	GTTGTAATGTGTTGATGTAACAGG	52	825	This study
	qnrS1_orf_r	CCCTATGTCTATTATTGCAAGG			This study