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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Immunol. 2014 Mar 21;192(9):4263–4272. doi: 10.4049/jimmunol.1303185

FIGURE 4.

FIGURE 4

Mtb impairment of DC activation and maturation requires MyD88- and TLR2/9-dependent pathways. Purified BMDCs from C57BL/6J and MyD88−/− (A) TLR2−/−(B) or TLR2/9−/− (C) mice were exposed to medium alone (ui) or infected with heat-killed wild type (wt) or the hip1 mutant at MOI=5 for 24 hours and cell-free supernatants were assayed for IL-12p40, IL-12p70 and IL-6 by ELISA. (D) Infected BMDCs from MyD88−/− and TLR2/9−/− were labeled with anti-CD11c-APC and anti-CD40-PE. Representative histograms and median PE Fluorescence intensity for CD11c+ cells is shown. Data are representative of three (A and B) or two (C and D) independent experiments. Values are presented as mean +/− SD, *, p<0.05; ***, p<0.001.