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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Immunol. 2014 Mar 21;192(9):4263–4272. doi: 10.4049/jimmunol.1303185

FIGURE 6.

FIGURE 6

Mtb-DC interactions modulate CD4 T cell differentiation in vitro and in vivo. (A) Purified C57BL/6J BMDCs in medium alone (ui) or infected with heat-killed wild type (wt) or the hip1 mutant at MOI=10 for 24h were co-cultured with CD4 T cells from C57BL/6J mice for 3 days. Cell-free supernatants were collected and assayed for IFN-γ and IL-17 by ELISA. (B) Single-cell suspensions were prepared from lungs of mice aerogenically infected with live wild type (wt) or the hip1 mutant at three weeks post infection, and cells were stimulated with 10 µg/ml ESAT-61–20 peptide for 48 hours. Supernatants were collected and assayed for IFN-γ and IL-17 by ELISA. Data are representative of three (A) or two (B) independent experiments. Values are presented as mean +/− SD. **, p<0.01; ***, p<0.001.