Skip to main content
. 2014 Apr 22;9(4):e95935. doi: 10.1371/journal.pone.0095935

Figure 3. TLR4 knockout weakens IL-1β, TNF-α, MCP-1 and MMP-2 release in primary SMCs.

Figure 3

The primary smooth muscle cells have been cultured from TLR4−/− and C57BL/6 mice. (A) The phenotype of TLR4−/− and C57BL/6 had been detected with Western-blot to assay TLR4 expression. Each type mice choose four different mice. (B) The cells have been incubated with or without oxLDL (50 ug/mL) for 48 hrs, and detected the level of IL-1β, TNF-α, MCP-1 and MMP-2 in the supernatant of cell culture medium. (Mean ± SD, n = 3;**P<0.01 compared with con group, $$P<0.01 compared with C57BL/6 mice group).