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. 2014 Mar 7;14:56. doi: 10.1186/1471-2180-14-56

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Copyright © 2014 Rüger et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Extracellular substrate and metabolite concentrations in pure and mixed cultures. Glutamine, glutamate and glucose were quantified by an enzymatic assay. Gluconate and 2-KDG were quantified by HPLC. Time courses of (A) glutamine (filled symbols) and glutamate (open symbols) and (B) glucose (half-filled symbols), 2-KDG (filled symbols) and gluconate (open symbols) are presented over a cultivation period of 12 h. For S. aureus neither gluconate nor 2-KDG were detected (n.d., below detection limit). Error bars represent standard deviation of three biological replicates.