Table 2. GM-CSF TALE target sites and RVD composition.
TALE | DNA Target Site | RVD Sequence | Length (bp) | % NI (A) | % NG (T) | % HD (C) | % NN (G) | Distance from lastRVD to TSS |
(Precluded by ‘T’) | ||||||||
G1 | CCTCTGTGTATTTAAGAGCT | HD HD NG HD NG NN NG NN NG NI NG NG NG NI NI NN NI NN HD NG | 20 | 20 | 40 | 20 | 20 | −19 |
G2 | AGTTCCCCCGCCTCCCT | NI NN NG NG HD HD HD HD HD NN HD HD NG HD HD HD NG | 17 | 6 | 24 | 58 | 12 | −69 |
G3 | GAAAACCCCCAAGCCT | NN NI NI NI NI HD HD HD HD HD NI NI NN HD HD NG | 16 | 38 | 6 | 44 | 12 | −157 |
G4 | CCAAACTGTGCCCCT | HD HD NI NI NI HD NG NN NG NN HD HD HD HD NG | 15 | 20 | 20 | 47 | 13 | −280 |
G5 | AGCCTGCCCAAAGGCCCCT | NI NN HD HD NG NN HD HD HD NI NI NI NN NN HD HD HD HD NG | 19 | 21 | 11 | 47 | 21 | −370 |
G6 | CCCCTCACACTCAAGTCT | HD HD HD HD NG HD NI HD NI HD NG HD NI NI NN NG HD NG | 18 | 22 | 22 | 50 | 6 | −467 |