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. 2014 Apr 22;9(4):e95913. doi: 10.1371/journal.pone.0095913

Figure 2. Left coronary artery ligation.

Figure 2

(A) Heart visualization by the 15 mm opening of the 5th intercostal space. (B) LAD was ligated with 4-0 silk suture and the plain knot was tied over two loops of suture. After 30′ ischemia loops were pulled and the knot was released allowing reperfusion.