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. 2014 Mar 6;11:43. doi: 10.1186/1742-2094-11-43

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Copyright © 2014 Minter et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Type-1 IFN production and signalling plays a role in the cellular response to OGD insult. M17 cells were subjected to 4.5 h of OGD and reperfused for a 0 to 24 h period. Q-PCR was then performed detecting: (A) IFNα and (B) IFNβ mRNA levels (***P <0.001, n = 3, unmatched one-way ANOVA, Dunnett’s post-hoc test). Western blot analysis was performed on total cell extracts probing for (C) P-Stat-1 and (D) P-Stat-3 and corresponding densitometry is shown (**P <0.01, n = 4, unmatched one-way ANOVA, Dunnett’s post-hoc test). For densitometry calculations, phosphorylation intensity was measured in arbitrary units (A.U.) and normalised to the β-tubulin loading control. Graphical data are represented as mean ± SEM.