Figure 1. C-di-AMP does not directly affect murine T cell proliferation in vitro.

Murine splenocytes were sorted for CD4⁺ and CD8⁺ T cells (CD62L^high, CD44^low, CD25⁻) and treated with either 5 µg/ml c-di-AMP, 5 µg/ml concanavalin A (ConA; positive control), or left untreated (control) in the presence of ³H-thymidine. As a measure for proliferation, ³H-thymidine incorporation in T cells was determined by scintillation. The error bars show SEM for n = 3. Differences were statistically significant at p<0.001 (***), p<0.05 (*) or non-significant (n. s.) with respect to the control.