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. 2014 Apr 22;9(4):e96091. doi: 10.1371/journal.pone.0096091

Figure 8. Effect of sesamin on NF-κB nuclear translocation and IκB-α phosphorylation induced by OVA.

Figure 8

(A) and (B) Immunoblotting detection of phosphorylated NF-κB in the nucleus (A), and phosphorylated and total IκB-α (B) in the cytosol in lung tissues from mice of sham group, OVA-sensitized and challenged mice treated with vehicle, or sesamin (1, 10, or 20 mg/kg b.w., i.p.). Increased nuclear translocation of phosphorylated NF-κB and increased IκB-α phosphorylation were observed in mice receiving OVA sensitization and challenge compared with mice receiving sham treatment. Levels of total IκB-α were significantly reduced in the OVA-stimulated mice. These changes were reversed in mice treated with 10 and 20 mg/kg but not with 1 mg/kg sesamin, compared with mice treated with vehicles. The quantitative results from three independent experiments were shown in (C)-(E). Each bar represents the mean ± S.E.M. Statistical significance was defined as ### p<0.01 compared with the sham group; and * p<0.05, ** p<0.01, *** p<0.001 compared with the OVA+vehicle group.