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. 2014 Mar 3;4:5. doi: 10.1186/2045-9912-4-5

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Copyright © 2014 Zhang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Short sevoflurane treatment increases the levels of P-GSK3β(ser9) and P-AKT(ser473) in H4 cells. A. Anesthesia with 4% sevoflurane for two hours increases the levels of P-GSK3β(ser9) in the H4 cells as compared to the control condition. There is no significant difference in β-Actin levels in the H4 cells between the sevoflurane anesthesia and control condition. B. Quantification of the Western blot shows that the sevoflurane anesthesia increases the levels of P-GSK3β(ser9) in the H4 cells as compared to the control condition. C. Anesthesia with 4% sevoflurane for two hours in H4 cells increases the levels of P-AKT(ser473) in the H4 cells as compared to the control condition. There is no significant difference in β-Actin levels in the H4 cells between the sevoflurane anesthesia and control condition. D. Quantification of the Western blot shows that the sevoflurane anesthesia increases the levels of P-AKT(ser473) in the H4 cells as compared to the control condition. P, phosphorylated; GSK3β, glycogen synthase kinase 3β. N = 6.