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. 2014 Feb 7;2:16. doi: 10.1186/2051-5960-2-16

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Copyright © 2014 Sokolowski et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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TubulinΔCsp6 highlights degenerating axon tracts during normal developmental pruning. Adjacent sections from embryonic day 14.5 mice were immunolabeled with CC3 (a), fractin (b) and tubulinΔCsp6 (cTub) (c) (green) along with neurofilament (2H3; red). All three antibodies reveal a subset of apoptotic cell bodies in the ganglion, but only cTub prominently labels axons emanating from the ganglia (c,d). Notice that cTub does not label any structures in adjacent non-neural somites (above and below axon tracts). Scale = 200 μm.