Figure 3.

The micro-pattern reduces biofilm formation of two key VAP-related pathogens, MRSA and P. aeruginosa when compared to un-patterned controls. The P. aeruginosa (ATCC 9027) strain did not form robust biofilms under these conditions, however the MRSA and the P. aeruginosa PA14ΔbifA strains showed 67% (p = 0.123) and 52% (p = 0.05) median reductions in biofilm compared to controls, respectively. A representative image per surface type for each organism was selected to reflect quantitative results. Images obtained by compiling the stack of images taken through the biofilm (Scale bar = 20 μm).