Table 2.
Renal functional parameters, markers of inflammation and oxidative stress
| S | I/R | I | EIso 1 ml/kg | EIso 2 ml/kg | EIso 4 ml/kg | |
|---|---|---|---|---|---|---|
| Cr (μmol/L) |
24 ± 5 |
98 ± 6* |
94 ± 15* |
96 ± 11* |
61 ± 6*†‡§ |
63 ± 3*†‡§ |
| BUN (mmol/L) |
6.5 ± 1.6 |
14.5 ± 4.7* |
13.9 ± 4.5* |
14.0 ± 3.5* |
9.3 ± 2.3*†‡§ |
9.6 ± 4.2*†‡§ |
| CYC (mg/L) |
1.35 ± 0.16 |
2.42 ± 0.27* |
2.5 ± 0.15* |
2.4 ± 0.24* |
1.71 ± 0.10*†‡§ |
1.87 ± 0.14*†‡§ |
| TNF-α (pg/ml) |
47.6 ± 1.0 |
121.0 ± 6.8* |
114.9 ± 10.1* |
120.2 ± 11.2* |
80.5 ± 5.4*†‡§ |
84.8 ± 7.7*†‡§ |
| IL-6 (pg/ml) |
36 ± 6 |
108 ± 9* |
98 ± 6* |
96 ± 4* |
67 ± 6*†‡§ |
71 ± 6*†‡§ |
| IL-10 (pg/ml) |
19 ± 6 |
57 ± 12* |
57 ± 11* |
67 ± 7* |
85 ± 10*†‡§ |
83 ± 7*†‡§ |
| MDA (nmol/mg) |
1.32 ± 0.19 |
2.05 ± 0.31* |
1.91 ± 0.35* |
1.98 ± 0.32* |
1.61 ± 0.28*†‡§ |
1.62 ± 0.17*†‡§ |
| SOD (U/mg) | 117 ± 10 | 78 ± 12* | 79 ± 13* | 80 ± 17* | 97 ± 7*†‡§ | 98 ± 10*†‡§ |
Serum concentrations of creatinine (Cr), blood urea nitrogen (BUN), cystatin c (CYC), tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-10, and renal tissue malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in rats in the sham (S), I/R, intralipid (I) and EIso groups (EIso 1, 2 or 4 ml/kg). Serum Cr, BUN, CYC, TNF-α, IL-6, IL-10 concentrations and renal tissue MDA content were significantly increased, and renal tissue SOD activity was markedly decreased after kidneys subjected to 45 min ischemia and 3 h reperfusion compared with the sham group. Compared with the I/R, intralipid and 1 ml/kg EIso groups, serum Cr, BUN, CYC, TNF-α, IL-6 levels and renal tissue MDA content were significantly reduced, and serum IL-10 level and renal tissue SOD activity were markedly increased after 2 or 4 ml/kg EIso pretreatment. Data represent mean ± SD. *P < 0.05 compared to S, †P < 0.05 compared to I/R, ‡P < 0.05 compared to I, §P < 0.05 compared to EIso 1 ml/kg.