Table 1.
Aβ-responsive genes in microglia for which EP4 signaling opposed the effect of Aβ by >1.5-fold
| RefSeq//gene symbol//gene assignment | Gene | Fold change | p | Fold change | p |
|---|---|---|---|---|---|
| Genes upregulated by Aß42 oligomers | Aß42o-veh versus Con-Veh | Aß42o + EP4 ag versus Aß42o-veh | |||
| NM_001033122 // Cd69 // CD69 antigen | Cd69 | 127.22 | 3.2E-06 | −1.92 | 1.6E-01 |
| NM_021274 // Cxcl10 // chemokine (C-X-C motif) ligand 10 | Cxcl10 | 24.75 | 5.5E-04 | −1.75 | 3.6E-01 |
| NM_138648 // Olr1 // oxidized low density lipoprotein (lectin-like) receptor 1 | Olr1 | 21.60 | 2.9E-09 | −1.89 | 4.0E-04 |
| NM_054055 // Slc13a3 // solute carrier family 13 | Slc13a3 | 19.03 | 2.1E-09 | −1.71 | 7.3E-04 |
| NR_027852 // Cd40 // CD40 antigen | Cd40 | 16.96 | 2.1E-11 | −2.69 | 8.8E-08 |
| NM_010260 // Gbp2 // guanylate binding protein 2 | Gbp2 | 16.91 | 2.3E-05 | −1.76 | 1.2E-01 |
| NM_153564 // Gbp5 // guanylate binding protein 5 | Gbp5 | 16.53 | 1.2E-06 | −1.54 | 8.1E-02 |
| NM_008204 // H2-M2 // histocompatibility 2, M region locus 2 | H2-M2 | 16.21 | 1.3E-10 | −1.73 | 3.8E-05 |
| NM_009890 // Ch25h // cholesterol 25-hydroxylase | Ch25h | 16.07 | 1.7E-08 | −2.52 | 7.2E-05 |
| NM_030701 // Niacr1 // niacin receptor 1 | Niacr1 | 15.24 | 8.4E-11 | −1.78 | 1.6E-05 |
| NM_172845 // Adamts4 // a disintegrin-like and metallopeptidase | Adamts4 | 11.24 | 3.3E-08 | −2.88 | 1.9E-05 |
| NM_008331 // Ifit1 // interferon-induced protein with tetratricopeptide repeats | Ifit1 | 10.35 | 2.9E-04 | −2.16 | 7.9E-02 |
| NM_172648 // Ifi205 // interferon activated gene 205 | Ifi205 | 10.23 | 3.2E-05 | −2.05 | 3.2E-02 |
| NM_008599 // Cxcl9 // chemokine (C-X-C motif) ligand 9 | Cxcl9 | 6.89 | 6.0E-07 | −3.97 | 7.8E-06 |
| NM_021384 // Rsad2 // radical S-adenosyl methionine domain containing 2 | Rsad2 | 6.84 | 2.7E-03 | −1.98 | 1.7E-01 |
| NM_008332 // Ifit2 // interferon-induced protein with tetratricopeptide repeats | Ifit2 | 6.46 | 3.5E-03 | −3.13 | 3.7E-02 |
| NM_010846 // Mx1 // myxovirus (influenza virus) resistance 1 | Mx1 | 6.43 | 4.1E-04 | −3.21 | 6.8E-03 |
| NR_029565 // Mir155 // microRNA 155 | Mir155 | 6.19 | 5.3E-09 | −1.59 | 1.8E-04 |
| NM_011909 // Usp18 // ubiquitin specific peptidase 18 | Usp18 | 5.89 | 4.0E-04 | −2.75 | 1.1E-02 |
| NR_003508 // Mx2 // myxovirus (influenza virus) resistance 2 | Mx2 | 5.77 | 1.1E-05 | −3.30 | 1.8E-04 |
| NM_029758 // Fam49a // family with sequence similarity 49, member A | Fam49a | 5.48 | 6.8E-07 | −1.70 | 2.5E-03 |
| NM_001045481 // Ifi203 // interferon activated gene 203 | Ifi203 | 5.46 | 9.2E-04 | −1.79 | 1.2E-01 |
| NM_020557 // Cmpk2 // cytidine monophosphate (UMP-CMP) kinase 2, mitochondrial | Cmpk2 | 5.40 | 2.8E-03 | −2.16 | 8.8E-02 |
| NM_018734 // Gbp3 // guanylate binding protein 3 | Gbp3 | 5.26 | 1.7E-05 | −2.22 | 2.4E-03 |
| NM_013652 // Ccl4 // chemokine (C-C motif) ligand 4 | Ccl4 | 5.00 | 2.7E-08 | −1.64 | 1.9E-04 |
| NM_029084 // Slamf8 // SLAM family member 8 | Slamf8 | 4.96 | 6.1E-07 | −2.02 | 2.6E-04 |
| NR_029806 // Mir221 // microRNA 221 | Mir221 | 4.86 | 8.5E-08 | −1.85 | 1.0E-04 |
| NM_001114665 // Fnbp1l // formin binding protein 1-like | Fnbp1l | 4.55 | 2.8E-09 | −1.87 | 2.8E-06 |
| NM_145545 // Gbp6 // guanylate binding protein 6 | Gbp6 | 4.25 | 3.1E-05 | −1.52 | 4.1E-02 |
| NM_145066 // Gpr85 // G protein-coupled receptor 85 | Gpr85 | 4.19 | 6.0E-09 | −1.57 | 4.1E-05 |
| NM_010657 // Hivep3 // human immunodeficiency virus type I enhancer binding pr | Hivep3 | 4.14 | 2.8E-08 | −1.77 | 3.1E-05 |
| NM_145968 // Tagap // T-cell activation Rho GTPase-activating protein | Tagap | 4.05 | 1.5E-09 | −1.57 | 1.0E-05 |
| NM_010104 // Edn1 // endothelin 1 | Edn1 | 3.91 | 1.6E-06 | −2.49 | 3.2E-05 |
| NM_008607 // Mmp13 // matrix metallopeptidase 13 | Mmp13 | 3.87 | 6.2E-07 | −1.82 | 2.5E-04 |
| NM_022331 // Herpud1 // homocysteine-inducible, endoplasmic reticulum stress-ind | Herpud1 | 3.68 | 1.6E-09 | −1.83 | 6.9E-07 |
| NM_001033415 // Shisa3 // shisa homolog 3 (Xenopus laevis) | Shisa3 | 3.63 | 2.9E-08 | −2.34 | 7.5E-07 |
| NM_010755 // Maff // v-maf musculoaponeurotic fibrosarcoma oncogene family | Maff | 3.54 | 1.0E-06 | −1.64 | 8.9E-04 |
| NM_001039530 // Parp14 // poly (ADP-ribose) polymerase family, member 14 | Parp14 | 3.38 | 2.4E-06 | −1.61 | 1.8E-03 |
| NR_029807 // Mir222 // microRNA 222 | Mir222 | 3.33 | 6.2E-07 | −1.74 | 1.9E-04 |
| NM_008390 // Irf1 // interferon regulatory factor 1 | Irf1 | 3.32 | 3.8E-07 | −1.63 | 2.7E-04 |
| NM_016850 // Irf7 // interferon regulatory factor 7 | Irf7 | 3.24 | 2.7E-03 | −1.56 | 1.4E-01 |
| NM_001042611 // Cp // ceruloplasmin | Cp | 3.21 | 5.0E-05 | −1.92 | 2.3E-03 |
| NM_013654 // Ccl7 // chemokine (C-C motif) ligand 7 | Ccl7 | 3.09 | 1.1E-04 | −1.95 | 3.0E-03 |
| NM_183177 // Zfp811 // zinc finger protein 811 | Zfp811 | 3.05 | 2.1E-07 | −1.70 | 5.6E-05 |
| NM_009425 // Tnfsf10 // tumor necrosis factor (ligand) superfamily, member 10 | Tnfsf10 | 2.98 | 2.0E-03 | −2.35 | 7.7E-03 |
| NM_008330 // Ifi47 // interferon gamma inducible protein 47 | Ifi47 | 2.96 | 6.8E-04 | −2.08 | 6.8E-03 |
| NM_181545 // Slfn8 // schlafen 8 | Slfn8 | 2.89 | 1.5E-05 | −1.73 | 1.4E-03 |
| NM_028968 // Ifitm7 // interferon induced transmembrane protein 7 | Ifitm7 | 2.88 | 4.1E-07 | −1.74 | 5.1E-05 |
| NM_001134457 // Fam55c // family with sequence similarity 55, member C | Fam55c | 2.87 | 2.9E-06 | −1.71 | 3.9E-04 |
| NM_027835 // Ifih1 // interferon induced with helicase C domain 1 | Ifih1 | 2.87 | 2.5E-06 | −1.82 | 1.5E-04 |
| NM_178607 // Rnf24 // ring finger protein 24 | Rnf24 | 2.84 | 2.5E-06 | −1.52 | 1.6E-03 |
| ENSMUST00000131035 // Rnf213 // ring finger protein 213 | Rnf213 | 2.82 | 1.9E-04 | −1.86 | 4.7E-03 |
| NM_011331 // Ccl12 // chemokine (C-C motif) ligand 12 | Ccl12 | 2.80 | 1.3E-06 | −2.76 | 1.5E-06 |
| NM_001037713 // Xaf1 // XIAP associated factor 1 | Xaf1 | 2.79 | 6.0E-05 | −1.88 | 1.5E-03 |
| NM_011333 // Ccl2 // chemokine (C-C motif) ligand 2 | Ccl2 | 2.78 | 5.9E-05 | −1.76 | 2.8E-03 |
| NM_023141 // Tor3a // torsin family 3, member A | Tor3a | 2.74 | 1.8E-05 | −1.76 | 9.6E-04 |
| NM_019440 // Irgm2 // immunity-related GTPase family M member 2 | Irgm2 | 2.66 | 2.7E-05 | −2.10 | 1.9E-04 |
| NM_009873 // Cdk6 // cyclin-dependent kinase 6 | Cdk6 | 2.61 | 5.7E-10 | −2.41 | 1.2E-09 |
| NM_194336 // Mpa2l // macrophage activation 2 like | Mpa2l | 2.60 | 3.2E-05 | −1.71 | 1.6E-03 |
| NM_183201 // Slfn5 // schlafen 5 | Slfn5 | 2.56 | 4.4E-05 | −1.80 | 1.1E-03 |
| NM_011579 // Tgtp1 // T-cell specific GTPase 1 | Tgtp1 | 2.56 | 1.3E-05 | −2.31 | 3.0E-05 |
| NM_023386 // Rtp4 // receptor transporter protein 4 | Rtp4 | 2.52 | 2.1E-05 | −1.62 | 1.7E-03 |
| NM_011579 // Tgtp1 // T-cell specific GTPase 1 | Tgtp1 | 2.50 | 1.1E-05 | −2.19 | 3.5E-05 |
| NM_009277 // Trim21 // tripartite motif-containing 21 | Trim21 | 2.48 | 2.5E-06 | −1.69 | 1.4E-04 |
| NM_183029 // Igf2bp2 // insulin-like growth factor 2 mRNA binding protein 2 | Igf2bp2 | 2.47 | 1.6E-07 | −1.62 | 1.9E-05 |
| NM_008326 // Irgm1 // immunity-related GTPase family M member 1 | Irgm1 | 2.42 | 3.9E-04 | −2.23 | 7.3E-04 |
| NM_021788 // Sap30 // sin3 associated polypeptide | Sap30 | 2.38 | 4.5E-06 | −1.75 | 1.1E-04 |
| NM_011057 // Pdgfb // platelet derived growth factor, B polypeptide | Pdgfb | 2.36 | 1.2E-05 | −2.65 | 4.6E-06 |
| NR_003507 // Oas1b // 2′-5′ oligoadenylate synthetase 1B | Oas1b | 2.35 | 5.7E-06 | −1.63 | 3.1E-04 |
| NM_010828 // Cited2 // Cbp/p300-interacting transactivator | Cited2 | 2.32 | 3.1E-06 | −1.75 | 6.3E-05 |
| NM_025992 // Herc6 // hect domain and RLD 6 | Herc6 | 2.31 | 2.4E-05 | −1.64 | 8.5E-04 |
| NM_008356 // Il13ra2 // interleukin 13 receptor, alpha 2 | Il13ra2 | 2.29 | 2.0E-07 | −1.72 | 4.9E-06 |
| NM_145636 // Il27 // interleukin 27 | Il27 | 2.25 | 4.4E-08 | −1.86 | 3.6E-07 |
| NM_010215 // Il4i1 // interleukin 4 induced 1 | Il4i1 | 2.21 | 3.0E-07 | −2.33 | 1.8E-07 |
| NR_029728 // Mirlet7c-1 // microRNA let7c-1 | Mirlet7c-1 | 2.15 | 5.6E-06 | −2.03 | 1.0E-05 |
| NM_013730 // Slamf1 // signaling lymphocytic activation molecule family member 1 | Slamf1 | 2.14 | 1.0E-06 | −1.59 | 4.3E-05 |
| NM_027320 // Ifi35 // interferon-induced protein 35 | Ifi35 | 2.14 | 2.6E-04 | −1.57 | 6.5E-03 |
| NM_172689 // Ddx58 // DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 | Ddx58 | 2.08 | 1.1E-04 | −1.66 | 1.3E-03 |
| NM_007829 // Daxx // Fas death domain-associated protein | Daxx | 2.07 | 1.3E-04 | −1.54 | 3.6E-03 |
| NM_008360 // Il18 // interleukin 18 | Il18 | 2.06 | 7.1E-05 | −2.07 | 6.7E-05 |
| NM_020583 // Isg20 // interferon-stimulated protein | Isg20 | 2.06 | 6.5E-04 | −1.53 | 1.3E-02 |
| NM_016960 // Ccl20 // chemokine (C-C motif) ligand 20 | Ccl20 | 2.05 | 7.1E-04 | −1.58 | 9.5E-03 |
| NR_029535 // Mir99a // microRNA 99a | Mir99a | 2.03 | 6.3E-06 | −1.87 | 1.6E-05 |
| Genes downregulated by Aß42 oligomers | |||||
| NM_008676 // Nbr1 // neighbor of Brca1 gene 1 | Nbr1 | −2.02 | 7.0E-09 | 1.51 | 4.7E-07 |
| NM_001037957 // Dyrk1b // dual-specificity tyrosine-(Y)-phosphorylation | Dyrk1b | −2.02 | 5.4E-08 | 1.90 | 1.2E-07 |
| NM_026875 // Ypel3 // yippee-like 3 (Drosophila) | Ypel3 | −2.04 | 2.3E-08 | 1.62 | 4.8E-07 |
| NM_011505 // Stxbp4 // syntaxin binding protein 4 | Stxbp4 | −2.05 | 6.9E-04 | 1.59 | 8.5E-03 |
| NM_021356 // Gab1 // growth factor receptor bound protein 2-associated protein 1 | Gab1 | −2.06 | 5.5E-07 | 2.82 | 3.4E-08 |
| NM_010591 // Jun // Jun oncogene | Jun | −2.08 | 6.2E-06 | 1.82 | 2.8E-05 |
| NM_007797 // Ctla2b // cytotoxic T lymphocyte-associated protein 2 beta | Ctla2b | −2.12 | 7.1E-07 | 2.16 | 5.9E-07 |
| NM_010847 // Mxi1 // Max interacting protein 1 | Mxi1 | −2.13 | 2.0E-08 | 3.92 | 1.8E-10 |
| ENSMUST00000162022 // Glis3 // GLIS family zinc finger 3 | Glis3 | −2.23 | 2.0E-05 | 1.65 | 5.4E-04 |
| NM_028149 // Fbxl20 // F-box and leucine-rich repeat protein 20 | Fbxl20 | −2.25 | 3.8E-08 | 1.82 | 3.9E-07 |
| NM_175445 // Rassf2 // Ras association (RalGDS/AF-6) domain family member 2 | Rassf2 | −2.26 | 2.0E-09 | 2.46 | 9.1E-10 |
| NM_025979 // Mastl // microtubule associated serine/threonine kinase-like | Mastl | −2.27 | 5.6E-04 | 1.60 | 1.4E-02 |
| NM_172589 // Lhfpl2 // lipoma HMGIC fusion partner-like 2 | Lhfpl2 | −2.32 | 1.1E-06 | 1.80 | 1.7E-05 |
| NM_133667 // Pdk2 // pyruvate dehydrogenase kinase, isoenzyme 2 | Pdk2 | −2.35 | 2.3E-06 | 2.09 | 7.0E-06 |
| NM_011454 // Serpinb6b // serine (or cysteine) peptidase inhibitor | Serpinb6b | −2.52 | 6.5E-07 | 1.70 | 4.0E-05 |
| NM_134250 // Havcr2 // hepatitis A virus cellular receptor 2 | Havcr2 | −2.61 | 4.6E-07 | 1.60 | 9.6E-05 |
| NM_021897 // Trp53inp1 // transformation related protein 53 inducible | Trp53inp1 | −2.74 | 7.8E-10 | 1.96 | 1.9E-08 |
| NM_007635 // Ccng2 // cyclin G2 | Ccng2 | −2.83 | 2.0E-06 | 1.89 | 7.7E-05 |
| NM_001081278 // Tbc1d4 // TBC1 domain family, member 4 | Tbc1d4 | −3.01 | 5.2E-08 | 1.63 | 2.7E-05 |
| NM_007901 // S1pr1 // sphingosine-1-phosphate receptor 1 | S1pr1 | −3.02 | 2.4E-07 | 2.60 | 7.6E-07 |
| NM_011050 // Pdcd4 // programmed cell death 4 | Pdcd4 | −3.28 | 2.9E-06 | 1.60 | 1.8E-03 |
| NM_133212 // Tlr8 // toll-like receptor 8 | Tlr8 | −3.55 | 3.5E-08 | 1.88 | 7.5E-06 |
| NM_145933 // St6gal1 // beta galactoside alpha 2,6 sialyltransferase 1 | St6gal1 | −3.59 | 7.4E-11 | 2.00 | 9.7E-09 |
| NM_146073 // Zdhhc14 // zinc finger, DHHC domain containing 14 | Zdhhc14 | −3.71 | 7.1E-09 | 2.39 | 1.8E-07 |
| NM_010658 // Mafb // v-maf musculoaponeurotic fibrosarcoma oncogene family | Mafb | −3.99 | 7.1E-10 | 2.09 | 1.0E-07 |
| NM_011882 // Rnasel // ribonuclease L | Rnasel | −4.29 | 1.0E-07 | 2.51 | 3.5E-06 |
| NM_175116 // Lpar6 // lysophosphatidic acid receptor 6 | Lpar6 | −5.30 | 1.6E-09 | 1.54 | 5.4E-05 |
| NM_009183 // St8sia4 // ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransf | St8sia4 | −5.53 | 1.9E-10 | 2.53 | 2.4E-08 |
| NM_009911 // Cxcr4 // chemokine (C-X-C motif) receptor 4 | Cxcr4 | −5.65 | 2.4E-12 | 1.74 | 2.0E-08 |
| NM_010427 // Hgf // hepatocyte growth factor | Hgf | −5.66 | 1.1E-09 | 1.72 | 9.7E-06 |
| NM_001042591 // Arrdc3 // arrestin domain containing 3 | Arrdc3 | −6.74 | 1.2E-08 | 1.95 | 3.8E-05 |
| NM_007564 // Zfp36l1 // zinc finger protein 36, C3H type-like 1 | Zfp36l1 | −8.01 | 7.3E-08 | 1.64 | 2.3E-03 |
| NM_146042 // Rnf144b // ring finger protein 144B | Rnf144b | −8.34 | 2.0E-11 | 1.70 | 1.1E-06 |