Figure 6.

Arp2/3 mediates actin polymerization, surface area expansion, and increase in number of filopodia in growth cones after NGF stimulation on laminin. a–d, E7 DRG growth cones on laminin, treated with NGF, and stained with phalloidin and antibodies against Arp3 and acetylated α-tubulin (Ac-Tubulin). e–h, E7 DRG growth cones on laminin treated with DMSO or CK666 for 4 h, stimulated with NGF or media as control, fixed, stained with Alexa Fluor 488 phalloidin, (i) and phalloidin fluorescent intensity quantified. j, Quantification of growth cone area. k, Average number of filopodia for every 100 μm of growth cone perimeter. Graphs show mean ± SEM.