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Arp2/3 inhibition reduces the area and number of paxillin-GFP puncta in growth cones on laminin. a–d, E7 DRG neurons were transfected with paxillin-GFP and mCherry-CA, grown overnight on laminin, and imaged through wide-field and TIRF microscopy. e, f, Thresholded TIRF images were used to measure the area and number of paxillin-GFP puncta.
