Table 1.

Arp2∕3 role in axon outgrowth rate and length

	Laminin	L1
Axon outgrowth ratea (μm∕h)
DMSO	20.68 ± 0.5881	15.26 ± 0.8684
CK666	18.02 ± 0.8024	10.76 ± 0.5519
Axon lengthb (μm)
DMSO	235.2 ± 11.88	198.2 ± 11.10
CK666	195.6 ± 12.61	153.0 ± 9.936
EGFP	236.8 ± 11.21	239.5 ± 13.39
EGFP-CA	182.2 ± 11.66	144.1 ± 12.01

^aMeasurements were done from time-lapse videos of E7 DRG explants' growth cones on L1 treated with DMSO or 50M CK666 4 h prior to imaging.

^bMeasurements were done on dissociated low-density cultures on L1 from E7 DRG neurons grown for 24 h, fixed, and stained with antibodies against neuronal β3-tubulin. For pharmacological inhibition of Arp2∕3 with CK666, neurons were allowed to adhere for 6 h before adding the drug.