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. 2014 Apr 23;34(17):6098–6106. doi: 10.1523/JNEUROSCI.4271-13.2014

Figure 2.

Figure 2.

Reduced amounts of TrkAP782S protein due to reduced mRNA levels. A, Reduced amount of TrkAP782S protein in KI mice. Lysates from cultured DRG neurons were subjected to Western blot analysis with the indicated antibodies. B, Quantification of TrkA protein levels in WT and KI DRG neurons. Western blots were scanned and quantified using ImageJ software. Data are presented as means ± SEM; p values were calculated using a two-tailed Student′s test (n = 12). C, qPCR analysis showing the reduced expression of trkAP782S mRNA. The trkA transcripts for WT and KI were 8683 ± 1123 and 5236 ± 314 per 100 ng of cDNA, respectively. Results are means ± SEM of three independent experiments performed in triplicate. The p value was calculated using a two-tailed Student's t test. D, Actin mRNA is not altered in KI DRG neurons. Actin transcripts for WT and KI were 103,700 ± 9707 and 116,500 ± 15,500 per 100 ng of cDNA, respectively. Results are means ± SEM of three independent experiments performed in triplicate. E, Increased protein/mRNA ratio in KI mice. The ratio of the amount of protein vs mRNA levels was normalized to the ratio of the WT sample. Note the increase in the ratio in the KI samples.