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. 2014 Apr 1;15:7. doi: 10.1186/1471-2091-15-7

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Copyright © 2014 de O Mello et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sequence map of the cloning of PvD₁into the expression vector pET-32 EK/LIC. The coding sequence of the PvD₁ (in bold) is shown linked to the LIC site of the vector pET-32 EK/LIC (indicated by a vertical arrow). The T7 promoter and terminator (horizontal arrows), lac operator, thioredoxin (Trx), six consecutive histidines (His) and (S) cleavable tags, thrombin and enterokinase protease sites, and His tag and terminator codon (-) are shown on the sequence map. The relevant amino acid sequences are shown below the nucleotide sequence. After expression and purification of the recombinant protein in this vector, all of the tags can be separated from the recombinant PvD₁ by a combined treatment with enterokinase as well as another chromatographic step.