Table 1.
Determination of LOH detection threshold by RFQ. Titrations series with artificial mixtures simulating (copy number neutral) loss of heterozygosity (LOH) in the HLA-A allele (for details see RFQ in material and methods).
| Percentage of heterozygous DNA (A*74/A*03) |
Percentage of homozygous DNA (A*03/A*03) |
Assumed ratio of alleles A*74 : A*03 |
Detected peak area ratio A*74 : A*03 |
|---|---|---|---|
| 100% | 0% | 1 : 1,00 | 1 : 1,04 |
| 90% | 10% | 1 : 1,22 | 1 : 1,23 |
| 80% | 20% | 1 : 1,50 | 1 : 1,47 |
| 67% | 33% | 1 : 2,00 | 1 : 1,89 |
| 50% | 50% | 1 : 3,00 | 1 : 2,94 |